BGC > Product Search > 5-Bromo-4-chloro-3-indolylphosphate p-toluidine salt, Reag.

Product Name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
CAS: 6578-06-9
MF: C8H6BrClNO4P.C7H9N
MW: 433.63
EINECS: 229-506-1
Product Categories: Indoles;Simple Indoles;substrate
Mol File: 6578-06-9.mol
mp 194-195℃
storage temp. 2-8°C
solubility DMF: 20 mg/mL
form tablets
CAS DataBase Reference 6578-06-9(CAS DataBase Reference)
EPA Substance Registry System 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1)(6578-06-9)
Safety Information
Hazard Codes Xi
Risk Statements 36/37/38-40
Safety Statements 26
WGK Germany 3
F 3-8-10
HS Code 29339190

Uses: alkaline phosphatase (alkaline phosphatase) of the histochemical substrate.
5-bromo-4-chloro-3-indole phosphoric acid (BCIP) and nitro-tetrazolium chloride (NBT)
were used for colorimetric identification of alkaline phosphatase-labeled molecules.
The BCIP / NBT substrate system is used in a wide range of applications including Northern Southern,
Western blotting, in situ hybridization and immunohistochemistry. BCIP may be in the form of two salts:
the disodium salt form is soluble in water and the p-toluidine form is soluble in dimethylformamide (DMF).
These salt forms can be used to prepare storage solutions and reaction buffers that bind to NBT,
constituting a substrate solution of alkaline phosphatase.
The substrate system was incubated with alkaline phosphatase to produce an insoluble NBT (diformazan) product with a purple color that was easily observed.