Description:
DAPI is a fluorescent DNA stain that binds to the minor groove of double-stranded DNA with preference for adenine and thymine (AT) rich DNA. DAPI is commonly used in microscopy and flow cytometry. It can be used on either fixed or live cells, although it crosses membranes in live cells less efficiently and demands higher concentrations to be used.
- Molecular Weight: 350.3
- Molecular Formula: C16H15N5·2HCl
Purity: ≥90%
Canonical SMILES:
C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
InChI:
InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChIKey: FPNZBYLXNYPRLR-UHFFFAOYSA-N
- Melting Point: >300°C (dec.)
- Solubility: H2O
- Appearance: Yellow Solid
- Application: Suitable for DNA staining in agarose gels, analysis of changes in DNA during apoptosis, detection of mycoplasma, photofootprinting of DNA, immunofluorescent staining of cells.
- Storage: Store at -20°C
Synonyms:
DAPI;2-[4-(aminoiminomethyl)phenyl]-1H-indole-6-carboximidamide dihydrochloride
More details are to be found on supplier website
