Description:
DAPI is a fluorescent DNA stain that binds to the minor groove of double-stranded DNA with preference for adenine and thymine (AT) rich DNA. DAPI is commonly used in microscopy and flow cytometry. It can be used on either fixed or live cells, although it crosses membranes in live cells less efficiently and demands higher concentrations to be used.
- Molecular Weight:350.3
- Melting Point:>300°C (dec.)
- Purity:≥90%
Molecular Formula:
C16H15N5·2HCl
Canonical SMILES:
C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl
InChI:
InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChIKey:
FPNZBYLXNYPRLR-UHFFFAOYSA-N
Solubility:
H2O
Appearance:
Yellow Solid
Application:
Suitable for DNA staining in agarose gels, analysis of changes in DNA during apoptosis, detection of mycoplasma, photofootprinting of DNA, immunofluorescent staining of cells.
Storage: Store at -20°C
Synonyms:
DAPI;2-[4-(aminoiminomethyl)phenyl]-1H-indole-6-carboximidamide dihydrochloride Más detalles se encuentran
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